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Not enough information. Need to know the shape of the data, and what kind of mathematical model (function) that you are using to fit the data. The model needs to be consistent with the observed data and expected model of inhibition. Are you fiitting a Dixon plot, uninhibited vs. inhibited hyperbolic data, Lineweaver-Burk plots, etc.? There are many models of inhibition, not all of which lead to 0% activity at saturating conversation.
Dear All,I am facing problem during a data interpretation of an Enzyme inhibitor kinetics experiments.I am using prism to calculate IC50 value for an Enzyme.Enzyme was almost inhibited (97%) at 600 microM inhibitor concentration.When I plot my data in prism the plot is looking fine but the IC50 value is very high and It is going out of range of my end concentration of inhibitor (which is 600 microM). if is it possible please explain? or am I dong something wrong?My inhibitor is a competitive inhibitor.Thanks in advance--WITH REGARDS
Rohit Kumar Singh