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Hi Hena, Tergazyme and 30 min in the sonicator bath followed by thorough rinsing works well for our high throughput operations. Cheers, Jan -- Beryllium Seattle Structural Genomics Center for Infectious Disease On Thu, Apr 7, 2016 at 9:12 AM, Andreas Forster <[log in to unmask]> wrote: > Dear Hena, > > I used Tergazyme with a passion. Eats all the crud from your loops and > makes them look like new. Wonderful stuff. Make sure to rinse properly > with distilled water afterwards to removed residual proteolytic activity. > > > http://www.sigmaaldrich.com/catalog/product/aldrich/z273287?lang=en®ion=US > > All best. > > > Andreas > > > > > On Thu, Apr 7, 2016 at 6:08 PM, Hena Dutta <[log in to unmask]> wrote: > >> Dear Members, >> Sorry for non-ccp4 question. Can someone tell which detergent I should >> use for cleaning cryo loop, used for crystal fishing? Or, someone can give >> a recipe for making the solution in house. >> Regards, >> Hena >> >> > -- Jan Abendroth Emerald Biostructures Seattle / Bainbridge Island, WA, USA home: Jan.Abendroth_at_gmail.com work: JAbendroth_at_embios.com http://www.emeraldbiostructures.com