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Hello Tim and Robert, Thanks for the suggestions. I will definitely give a try on both. Meanwhile, do you have any suggestions for an alternative to RCrane? We do not work on RNA very often but would for sure like to know more on that. Thanks a lot! Sam Biochemistry Programme, School of Life Sciences, CUHK On 23 April 2016 at 03:35, Tim Gruene <[log in to unmask]> wrote: > -----BEGIN PGP SIGNED MESSAGE----- > Hash: SHA1 > > Dear Sam, > > as far as I know, RCrane has not been developed for 4 years or more. Kevin > Keating had already taken up a new position when I worked on the knuspr GUI > interface to RCrane back in 2012. > > I guess there are naming discrepancies between the RCrane code and the PDB > standards used by Refmac5. > > You might get away with fixing the RCrane code for the atom names or RNA > residue names without breaking it. Make sure you have a backup, though. > > Best regards, > Tim > > On Friday, April 22, 2016 02:16:03 PM Sam Tang wrote: > > Hi again > > > > I am wondering if anyone has the experience using a plugin in Coot called > > RCrane to build RNA and then refine with Refmac. It happens that refmac > > cannot recognize my di-/tri-nucleotide and complains of missing atoms. No > > problem for single nucleotides built from the Coot library. > > > > Would be glad if anyone with similar experience could share further on > > this. > > > > Thanks in advance. > > > > Kind regards > > > > Sam > > > > Biochemistry Programme, School of Life Sciences, CUHK > - -- > - -- > Paul Scherrer Institut > Tim Gruene > - - persoenlich - > OFLC/102 > CH-5232 Villigen PSI > phone: +41 (0)56 310 5297 > > GPG Key ID = A46BEE1A > -----BEGIN PGP SIGNATURE----- > Version: GnuPG v2 > > iD8DBQFXGnz4UxlJ7aRr7hoRAoGtAJ4xt+GPojZIviBdaMMOQsuuIo/cIQCggfAm > GLr2Z5CCs+fxMm5m34gfO7c= > =upQ2 > -----END PGP SIGNATURE----- > >