Hi FSL peeps, I was hoping to get the total brain volume for a subject, which includes WM, GM and perhaps CSF (?) I am looking into FAST, which does a segmentation of these 3 tissue types: http://fsl.fmrib.ox.ac.uk/fsl/fslwiki/FAST#Tissue_Volume_Quantification Here it is explained how to estimate the tissue volume for a given class, as such: vol=`$FSLDIR/bin/fslstats structural_bet_pve_1 -V | awk '{print $1}'` mean=`$FSLDIR/bin/fslstats structural_bet_pve_1 -M` tissuevol=`echo "$mean * $vol" | bc -l` echo $tissuevol My question is - how can I calculate the TOTAL brain volume? Can I do this simply by summing the tissuevol of the WM, the GM, and the CSF? Furthermore, is it typical to include the CSF in the total brain volume? Thanks, Jacqueline