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-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Z, naively I would think that rigid body refinement, maybe with ncsr switched on in refmac, is similar if not superior to improving NCS operators based on the density. Then you can click on Calculate->NCS map in coot after you read in the PDB file. Best, Tim On Friday, March 04, 2016 01:44:00 PM Zachary A. Wood wrote: > Hello All, > > Does anyone know of any ‘newer’ programs that do what Kleywegt’s wonderful > RAVE software package does? > > What is great about RAVE is that you generate a mask based on the > coordinates of a protomer, you then optimize your NCS operators using the > masked electron correlation coefficients of the other protomers, and then > generate a nice, averaged electron density map for model building. The > electron density based optimization of the NCS operators is the best > feature in my opinion. I am hoping that someone has automated this process > of electron density averaging. Don’t get me wrong, RAVE does a fantastic > job, but it is a bit time consuming and seems ripe for some automation > (especially when teaching undergrads how to do this stuff). Thanks in > advance for any suggestions. > > > Best regards, > > Z > > > *********************************************** > Zachary A. Wood, Ph.D. > Associate Professor > Department of Biochemistry & Molecular Biology > University of Georgia > Life Sciences Building, Rm A426B > 120 Green Street > Athens, GA 30602-7229 > Office: 706-583-0304 > Lab: 706-583-0303 > FAX: 706-542-1738 > *********************************************** - -- - -- Paul Scherrer Institut Tim Gruene - - persoenlich - OFLC/102 CH-5232 Villigen PSI phone: +41 (0)56 310 5754 GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v2 iD8DBQFW2ZTHUxlJ7aRr7hoRAkc5AKCJUYCXR62QgBSS+WmppkA7c8+nlgCgvyFv GuHkEkJAK9eb/QFOpAoZl2w= =ZYn3 -----END PGP SIGNATURE-----