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Sjors For economy of time I have been doing all the processing of single particles with bin2 images, and after a 3D classification run, wanted then to use a particular class and run a 3D refinement with the bin1 (ie full sized) particles. I wrote a script to update the micrograph/particle names, to update x,y, magnification and 3 defocus parameters in the existing 3D-class.star file to represent the full sized particles, and then wrote out a new star with the same header format. However, I get a strange scaling error I have not encountered before, but note that I did normalize the full size particles when they were extracted from their micrographs. At the first Expectation step the job stalled and a file appeared in the working dir > Mweight.spi The cluster slowed down to a load near zero, and the .err output file contains a very long list of 0 values and a few comments at the top: exp_thisparticle_sumweight= 0 part_id= 64278 group_id= 8 mymodel.scale_correction[group_id]= 1 exp_ipass= 0 sampling.NrDirections(0, true)= 192 sampling.NrDirections(0, false)= 192 sampling.NrPsiSamplings(0, true)= 24 sampling.NrPsiSamplings(0, false)= 24 mymodel.sigma2_noise[ipart]= qq at the tail: mymodel.avg_norm_correction= 1 wsum_model.avg_norm_correction= 0.868591 written out Mweight.spi exp_thisparticle_sumweight= 0 exp_min_diff2[ipart]= 738.506 ERROR!!! zero sum of weights.... File: src/ml_optimiser.cpp line: 4239 In thread 0 Is there another set of params I should have updated or deleted in the 3D-class.star file? thanks, C Akey