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Hi, We have an E. coli codon optimized gene that we would like to try expressing in insect cells. The protein is not expressing and we are concerned that it may be because of the codon optimization. Could this be a possibility, or are the insect optimized codons similar to that of E. coli? Thanks. Best wishes, Reza Reza Khayat, PhD Assistant Professor City College of New York Department of Chemistry New York, NY 10031