Dear Colleagues,
We are attempting to express our target protein fused to MBP in BL21(DE3) and BL21(RIPL). Unfortunately, we get a mixture of MBP-target protein and MBP only, which severely depletes our overall yield of MBP-target protein. We've tried enriched media, such as
2xYT & TB and also optimal high-density induction in minimal media at temperatures ranging from 20-37deg and IPTG from 0.05-0.5microM.
Any suggestions to address this competitively high yield of MBP-only protein?
Cheers,
Elias
