Dear all,
I'm currently working with a protein that I can't solve using SeMet substitution (the ~220 aa protein only has 3 Met and two of them are likely to be disordered as they are part of the His-tag and the protein's start codon). So I'm setting up some heavy atom soaks. the problem I have is that the best crystals I've obtained so far grow in TRIS pH=9. As far as I know, this pH is less than ideal for Hg, Pt, Sm and Lu, which are my heavy metals of choice.
Could you recommend any heavy atoms that would work at this pH?
Cheers,
Tony
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Dr. Antonio Ariza
University of Oxford
Sir William Dunn School of Pathology
South Parks Road
Oxford
OX1 3RE