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I would try matrix seeding with the crystals – make a seedstock with your stacked crystals, and set up an initial crystallization screen,
including not only protein and crystallant but also 10% or so of the seedstock – there is a recent paper on how to do this. this is a powerful technique, but depending on what the initial crystals grow in you can get a lot of salt crystals
Cheers, Janet
D’Arcy, A., Bergfors, T., Cowan-Jacob, S. W. & Marsh, M. (2014).
Acta Crystallographica Section F Structural Biology Communications. 70, 1117–1126.
From: CCP4 bulletin board [mailto:[log in to unmask]]
On Behalf Of monika chandravanshi
Sent: Monday, 29 February 2016 5:11 PM
To: [log in to unmask]
Subject: [ccp4bb] Thin stacked plate crystal
Dear All,
We are working on a protein (43kDa), which forms plate-like crystals in several crystallization conditions. We also observe that in sitting-drop, crystals are small but single. However, when we set up the
crystallization in hanging-drop, crystals are bigger but stacked. We have tried crystallizing the protein in different protein:buffer ratios (2:1, 2:2, 3:1). These crystals diffract to a low resolution (between 3 to 4 Angst).
My question is how to obtain single and bigger crystals?
The pictures of initial crystals are attached herewith for your kind reference.
Thanking you all.