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Hi FSL experts, I performed TBSS as described in FSL course manual and the papers. When I read your paper (Smith et al, 2006), I have a one question. Why would you select 0.3-0.6 as FA for making skeleton in your course manual? There was discription about the lower limit of skeleton avoiding CSF or gray matter but not the upper limit. I think that the body/genu of corpus callosum and the posterior limb of internal capsule can show 0.6-0.9. I should not observe the posterior limb of internal capsule nor c.c. when I adopt 0.3-0.6, but I may get the result in the body of the c.c. from ANOVA after TBSS. I may have read the previous mailing list that someone used high upper limit. But TBSS had not work with high upper limit. I might have read in some paper or manual indictated that the high upper limit induced some influence for skeleton. I do not fully remember the upper limit selecting FA for making skeleton and can not find the good paper. I am grateful if you would tell me about the reason that you selected the upper FA limit for skeleton. Best regards, S Fujii