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Dear all,
I have a question on how to improve the resolution of protein crystals. I am working on a protein which crystallizes in the following conditions
1) 0.1M Magnesium nitrate hexahydrate & 10% PEG 3350
2) 0.2M Calcium chloride dihydrate & 6-14% PEG 3350
3) 0.2M Calcium acetate hydrate & 6-14% PEG 3350
The crystals diffracted to a very
low resolution (~ 4 Angst, kindly see the attached image) at home source. To
prevent ice formation, different cryoprotectants such as glycerol,
2-methyl-2,4-pentanediol, PEG 400, PEG 3350, Hexanediol, were used.
Any suggestion to improve the x-ray diffraction resolution will greatly help
me.
Thanking you.
Prerana