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Faisal, BMe, DTT, sulfites, cysteine and TCEP are commonly used reducuing agents. In a vapour diffusion set-up, where air (and oxygen) is present, the protection these offer has an expiration date. Hence, to prolong the effect, DTT/TCEP or other non-volatile agent is used in the protein drop and a volatile agent like BMe is used in much higher concentrations in the reservoir. Thus after the reservoir solution is mixed with the protein drop, an extra amount of BMe is added to the reservoir. The concentration needed will vary depending on the propensity of the protein to oxydize. In extreeme cases, experiments must be done in an anerobic glove box. I hope this helps, Enrico. On Mon, 25 Jan 2016 21:31:28 +0100, khaja faisal tarique <[log in to unmask]> wrote: > Dear > > Sorry for a redundant and a trivial question but among BMe, DTT and TCEP > which is the best reducing agent for crystallization purpose ? Can > replacing one with other affects the crystallization process > significantly > ? Does the preference varies when you are going for the protein > complexes ? > What is the optimum concentration for each to use keeping in mind the > half > lives of each ? > > Regards > > Faisal -- Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71 Proxima-2A, Soleil Synchrotron. Tel: 33 (0)1 69 35 8180 Beamline http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate