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Dear experts Thank you so much for your all help during your FSL course. I would like to ask you a question about Single-Group Average with Additional Covariate on FSLwiki, GLM. On FSLwiki, I read that how to use one-sample T test is only for fMRI data, which was restricted. However, there is a few paper about the association when they use TBSS recently. Also, when I had read this mailing list, someone might have asked a question about multiple regression for TBSS and written the script. And a user might upload the scripts as multiple regression for TBSS. So, I have analyzed FA and physiological data in healthy participants using ANOVA for devided tertile of physiological data and onesample T test with physiological data as EV1 (contrast: 1, line break, -1) like mean activation on Single-Group Average with Additional Covariate, GLM, FSLwiki. My results using these two methods overlapped partially. Is the latter analysis wrong? Or is the methods using Monte Carlo test MCMC-regression? I can not fully understand the permutation. That is why I will select the former results using ANOVA. Do you have a way to identify association or correlation when I use TBSS, FA and physiological data in healthy participants? I would be grateful if you could help me. S Fujii