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Hello all, I'm going through the relion tutorial and trying to complete the *Extracting and normalising particles *step. In the previous *Manual particle picking* procedure, I used *e2boxer.py *to pick and generate coordinate (.box) files. Out of 571 micrographs, I picked from 19 to get ~3000 to use as my class 2d average template. In my current efforts to extract and normalise particles, I'm following the tutorial closely except deviating from the *Coordinate-file suffix* and *Particle box size *parameters. Otherwise everything else is identical. But when I try to run the extraction process via the gui or script, I get File: src/image.cpp line: 80 -------------------------------------------------------------------------- MPI_ABORT was invoked on rank 14 in communicator MPI_COMM_WORLD with errorcode 1. NOTE: invoking MPI_ABORT causes Open MPI to kill all MPI processes. You may or may not see output from other processes, depending on exactly when Open MPI kills them. -------------------------------------------------------------------------- When the run completes, I get a new *Particles *directory (not */Particles/Micrographs*) with directories for each of the 19 coordinate files (all of which are empty). Additionally, I don't get a *particles_manual.star *output file despite naming it in the *Extract rootname* (which backs up the idea that nothing is getting extracted). Perhaps the MPI error is a server-related issue but I wanted to ask. And as the other hiccups are concerned (e.g., missing particles_manual.star, etc) are these a consequence of using e2boxer.py and missing an intermediate step before attempting an extraction? Thank you very, very much for you help! (P.S., perhaps it would behoove me to note that I also get warning messages for "missing coordinate files" of the micrographs I *haven't* picked Though I would fully expect that, perhaps this is a sources of error that I've overlooked). -- Best, Breylon Riley