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Hi Antje, Have you checked this page of relion wiki? http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Grouping_procedure It describes scripts (no GUI) that group particles based on the criterion of your choice. Best Ben De : "[log in to unmask]<mailto:[log in to unmask]>" <[log in to unmask]<mailto:[log in to unmask]>> au nom de "Aufderheide, Antje" <[log in to unmask]<mailto:[log in to unmask]>> Répondre à : "Aufderheide, Antje" <[log in to unmask]<mailto:[log in to unmask]>> Date : lundi, 23 novembre 2015 10:38 À : "[log in to unmask]<mailto:[log in to unmask]>" <[log in to unmask]<mailto:[log in to unmask]>> Objet : Re: [ccpem] grouping large data sets Hi Ludo, thank you for your reply! I had actually tried what you suggest, but as far as I understand you can only save a new star file with the grouped particles, if you select all the displayed particles and then click "save selected particles to star file" and this is the point, where relion breaks down. Therefor, the gui itself is not the problem. So I am looking for a way to use the grouping procedure implemented in RELION without displaying the particles or a way to save displayed particles different from the way mentioned above. Cheers, Antje ------------------------------------- Antje Aufderheide, Dipl. Phys. PhD candidate Max Planck Institute for Biochemistry Dept. Molecular Structural Biology Am Klopferspitz 18 82152 Martinsried ________________________________ From: Ludovic Renault [[log in to unmask]<mailto:[log in to unmask]>] Sent: Friday, November 20, 2015 4:16 PM To: Aufderheide, Antje Cc: [log in to unmask]<mailto:[log in to unmask]> Subject: Re: [ccpem] grouping large data sets Hi, relion_display can be run without gui just type relion_display in a terminal and press enter and you list all the options. There's a --regroup option. I guess that's what you want. Cheers, Ludo On Fri, Nov 20, 2015 at 3:08 PM, Aufderheide, Antje <[log in to unmask]<mailto:[log in to unmask]>> wrote: Hi, I have a big dataset to process. It contains a lot of particles but not many particles per micrograph, so I have to group them. I tried to use the grouping procedure via relion_display, it is working fine until I want to save the selected (all) particles to a star file. Then the program doesn't react any more, i.e. doesn't give me the window to in which you give the star file name. It works fine on a smaller subset of the same data set. Is there a way to use the grouping procedure implemented in the display function without actually displaying the particles? Or should I go back to the "old" procedure and group the particles/ micrographs by defocus values? Or should I rather process the dataset in subsets? Any suggestions would be highly appreciated! Many thanks! Antje ------------------------------------- Antje Aufderheide, Dipl. Phys. PhD candidate Max Planck Institute for Biochemistry Dept. Molecular Structural Biology Am Klopferspitz 18 82152 Martinsried