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Dear all I have been trying to crystallize an 11 kDa protein. After few days (approx. a week) of setting up the crystallization, some gel-like or oily appearance (see attachment) is observed. Even after a couple of month or more, these oily appearance persists and does not lead to any crystal formation. The buffer conditions in which this kind of appearance occurs mostly contain one common chemical i.e. Sodium acetate trihydrate. I have tried changing the pH of the buffer and also the temperature conditions, however, I have not observed any difference. What else can I do? Please help. Thank you! Prerana