On 6 Oct 2015, at 7:00 pm, Bernhard Rupp (Hofkristallrat a.D.) <[log in to unmask]> wrote:

Dear Lepidopteranites,

I wonder if someone could educate me whether it is meaningful to produce

insect cell media in-house. My fellows found the SF900 III medium optimal

for our glycoprotein, but yields are still modest and bankruptcy is near.

I found this review and compilation

http://link.springer.com/protocol/10.1007%2F978-1-59745-457-5_8

SF900 III SFM is a “optimized low-hydrolysate, serum-free, protein-free, animal origin–free

insect cell culture medium” but as the review states:

“The lack of information concerning the detailed composition of commercial

serum-free media is often a limitation“. No kidding.

Does anyone have experience/feasibility to share? It seems quite elaborate, but

might still be cost effective over time.

Thx, BR

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Bernhard Rupp

Innsbruck Medical University

Schöpfstrasse 41

A 6020 Innsbruck – Austria

+43 (676) 571-0536

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Crystallographiae Vindicis Militum Ordo

Vista, CA 92084

001 (925) 209-7429

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http://www.ruppweb.org/

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