Dear Monica,

I feel your pain :) However, do not give up on HA quite yet. There are literally thousands of options, including Iodine (which, if done right, is quite gentle!) and various 'weird' rare earths (like Samarium, Europium, or my recent favorite Praseodymium) -- point being if you have diffracting crystals you are 90% there, and the last 10% is worth the effort.

Please feel free to contact me personally if you would like some specific advice. Even though I run a company now, we are always available (and always free) for advice to our academic colleagues.

Artem

www.harkerbio,com

On Mon, Oct 5, 2015 at 3:38 AM, Monica Mittal <[log in to unmask]> wrote:

Dear all,

I need a general advice. I don't have a suitable Model (Lets say only with a similarity of 15%) and no other model to solve the structure by MR. Then i think of anomalous experiments, but soaking with heavy metals is not good for the crystals. Since the protein is being expressed in Pichia cells, growth in Seleno-media is cumbersome. There are no ligands or ions that i can use for anomalous scattering of protein crystals. I am trying to express it in bacteria but have some issues. What is the option that i can choose to solve the structure of such a protein ? Your suggestions are highly recommended.

Thanks in advance.
Monica