HI,

Though there is barely any sequence similarity but if the protein has some part which is structurally homologous to other, then you can use small fragment of structure to phase it by MR programs. MRage in PHENIX worked for one of the structure for us. You can build homology model and try it or or part of it for MR phasing. How many molecules are there in ASU?

On 5 October 2015 at 01:38, Monica Mittal <[log in to unmask]> wrote:

Dear all,

I need a general advice. I don't have a suitable Model (Lets say only with a similarity of 15%) and no other model to solve the structure by MR. Then i think of anomalous experiments, but soaking with heavy metals is not good for the crystals. Since the protein is being expressed in Pichia cells, growth in Seleno-media is cumbersome. There are no ligands or ions that i can use for anomalous scattering of protein crystals. I am trying to express it in bacteria but have some issues. What is the option that i can choose to solve the structure of such a protein ? Your suggestions are highly recommended.

Thanks in advance.
Monica