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Try Wide Search Molecular Replacement (WSMR) at https://portal.sbgrid.org/d/apps/wsmr/ HTH Kay On Mon, 5 Oct 2015 09:38:54 +0200, Monica Mittal <[log in to unmask]> wrote: >Dear all, > >I need a general advice. I don't have a suitable Model (Lets say only with >a similarity of 15%) and no other model to solve the structure by MR. Then >i think of anomalous experiments, but soaking with heavy metals is not good >for the crystals. Since the protein is being expressed in Pichia cells, >growth in Seleno-media is cumbersome. There are no ligands or ions that i >can use for anomalous scattering of protein crystals. I am trying to >express it in bacteria but have some issues. What is the option that i can >choose to solve the structure of such a protein ? Your suggestions are >highly recommended. > >Thanks in advance. >Monica >