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I meant Soleil, apologies > On 5 Oct 2015, at 09:58, Phil Evans <[log in to unmask]> wrote: > > Xe at longish wavelength (> 1.5Å), if you can find a pressure device (LURE maybe?) > > Phil > >> On 5 Oct 2015, at 09:08, Marc Graille <[log in to unmask]> wrote: >> >> Dear Monica, >> >> I had solved couple of structures using anomalous signal for tungsten. I have incubated the proteins with 25-50mM NaWO3 and set-up the crystallization trays. >> If you are lucky, WO3 might interact with your protein as SO4 ions do sometimes and you might be able to get anomalous signals. >> This works fine for nucleic acid binding proteins. >> >> Otherwise, I have also solved a structure of a protein expressed in Pichia using anomalous signal from SeMet. It might be time-consuming and complicated to get labeled protein in Pichia but could be faster than heavy atoms derivatives. >> Here is a link to the publication describing the SeMet labeling procedure. >> http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/26082394 >> >> Hope it helps, >> >> Good luck, >> >> Marc >> >>> Le 5 oct. 2015 à 09:38, Monica Mittal <[log in to unmask]> a écrit : >>> >>> Dear all, >>> >>> I need a general advice. I don't have a suitable Model (Lets say only with a similarity of 15%) and no other model to solve the structure by MR. Then i think of anomalous experiments, but soaking with heavy metals is not good for the crystals. Since the protein is being expressed in Pichia cells, growth in Seleno-media is cumbersome. There are no ligands or ions that i can use for anomalous scattering of protein crystals. I am trying to express it in bacteria but have some issues. What is the option that i can choose to solve the structure of such a protein ? Your suggestions are highly recommended. >>> >>> Thanks in advance. >>> Monica >> >> Marc GRAILLE, PhD >> Directeur de recherche CNRS >> >> Laboratoire de Biochimie >> ECOLE POLYTECHNIQUE - UMR7654 CNRS >> 91128 PALAISEAU CEDEX >> >> <Signature-email.jp2> >> >> Phone : +33 (0)1 69 33 48 90 – Fax : +33 (0)1 69 33 49 09 >> >> Email: [log in to unmask] >> >> Team: Translation and degradation of eukaryotic mRNAs >> http://bioc.polytechnique.fr/spip.php?rubrique117 >> Team supported by the ATIP-Avenir CNRS program >> >> >>