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Hi Anderson, Thank you very much for this great explanation! Please I have one more question: After using FAST to do segmentation for the white matter. Is it correct to register this segmented white matter to MNI space then merge the images and run randomise? Actually, what I am aiming for is to have at the end statistical overlay on top of MNI to see changes in white matter volume WMV ( Exactly like in TBSS analysis where we see the changes in FA on skeleton but the difference her I will not visualize the changes in WMV on a skeleton ) Thanks for any suggestion