HI All, I'm fairly new to Tracula but am getting the error message seen way below here when I run the trac-prep script. It seems that it has a problem with opening the bvals file within the dmri folder that it creates (the values seem to be changed back to rows instead of columns) unlike what we created in the testbvals file. This does not seem to be a problem with my machine or with permissions since I can open the bvals file within dmri so I think it may be an issue with the actual trac -prep script. The complete log of the processing and the error message is below. I'm also attaching the trac-prep script and the bvals file it creates within the dmri folder, along with the original testbvals file that we used in our drmrirc file. The trac-prep script also creates two folders: dlabel and dmri. 1)Within dlabel is the diff folder within which we only get the lowb_brain_mask file 2) Within dmri, we get an empty xfms folder as well as the following files: bvals, bvecs, bvecs.norot, data.nii.gz, dcminfo.dat, dwiecclog, dwi.nii.gz, dwi_orig.mghdti.bvals, dwi_orig.mghdti.bvecs, dwi_orig.nii.gz, dwi_orig_fli-p.nii.gz, lowb.nii.gz, lowb_brain.nii.gz Just checking to see if trac -prep is supposed to create any other files? Thanks so much! We appreciate any help you can give us with this. Cheers, -Karuna INFO: SUBJECTS_DIR is /home/karunas/Desktop/bin/freesurfer/subjects INFO: Diffusion root is /home/karunas/Desktop/bin/freesurfer/subjects Actual FREESURFER_HOME /home/karunas/Desktop/bin/freesurfer ERROR: cannot find /home/karunas/Desktop/bin/freesurfer/subjects/203 INFO: SUBJECTS_DIR is /home/karunas/Desktop/bin/freesurfer/subjects INFO: Diffusion root is /home/karunas/Desktop/bin/freesurfer/subjects Actual FREESURFER_HOME /home/karunas/Desktop/bin/freesurfer trac-preproc -c /home/karunas/Desktop/bin/freesurfer/subjects/203/scripts/dmrirc.local -log /home/karunas/Desktop/bin/freesurfer/subjects/203/scripts/trac-all.log -cmd /home/karunas/Desktop/bin/freesurfer/subjects/203/scripts/trac-all.cmd #------------------------------------- /home/karunas/Desktop/bin/freesurfer/bin/trac-preproc #------------------------------------- #@# Image corrections Wed Sep 23 12:33:46 PDT 2015 mri_convert /data/bil-mb7/fMRI/MESSI/HC2/testtrac/203/E1S16I1.dcm /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz mri_convert /data/bil-mb7/fMRI/MESSI/HC2/testtrac/203/E1S16I1.dcm /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz $Id: mri_convert.c,v 1.213 2014/07/29 19:22:31 fischl Exp $ reading from /data/bil-mb7/fMRI/MESSI/HC2/testtrac/203/E1S16I1.dcm... crypt_gkey = FSre8SL5LUSmU Getting Series No INFO: Found 61 files in /data/bil-mb7/fMRI/MESSI/HC2/testtrac/203 INFO: Scanning for Series Number 16 Scanning Directory INFO: found 35 files in series INFO: loading series header info. RunNo = 15 WARNING: Run 1 appears to be truncated Files Found: 35, Files Expected (lRep+1): 1 FileName /data/bil-mb7/fMRI/MESSI/HC2/testtrac/203/E1S16I1.dcm Identification NumarisVer syngo MR B15 ScannerModel TrioTim PatientName vinlab_ER_4_8_2011 Date and time StudyDate 20110408 StudyTime 162622.453000 SeriesTime 180250.125000 AcqTime 175951.002500 Acquisition parameters PulseSeq *ep_b0 Protocol dti_35dir PhEncDir COL EchoNo 1 FlipAngle 90 EchoTime 84 InversionTime -1 RepetitionTime 9000 PhEncFOV 256 ReadoutFOV 256 Image information RunNo 15 SeriesNo 16 ImageNo 1 NImageRows 1152 NImageCols 1152 NFrames 35 SliceArraylSize 72 IsMosaic 1 ImgPos 1012.3245 1267.7782 -174.7016 VolRes 2.0000 2.0000 2.0000 VolDim 128 128 72 Vc -0.9892 -0.0962 0.1107 Vr 0.0985 -0.9950 0.0158 Vs 0.1086 0.0266 0.9937 VolCenter 0.0000 0.0000 0.0000 TransferSyntaxUID 1.2.840.10008.1.2.2 INFO: sorting. INFO: (128 128 72), nframes = 35, ismosaic=1 Could not parse NUMARIS version string syngo MR B15 found in dicom tag 18,1020 (len = 3 != 6) Repetition Time = 9000, TR = 9000 ms PE Dir COL COL AutoAlign matrix detected AutoAlign Matrix --------------------- 0.98919 -0.12584 -0.07527 8.62242; 0.09619 0.94432 -0.31466 -12.16901; 0.11068 0.30402 0.94621 25.01079; 0.00000 0.00000 0.00000 1.00000; FileName /data/bil-mb7/fMRI/MESSI/HC2/testtrac/203/E1S16I1.dcm Identification NumarisVer syngo MR B15 ScannerModel TrioTim PatientName vinlab_ER_4_8_2011 Date and time StudyDate 20110408 StudyTime 162622.453000 SeriesTime 180250.125000 AcqTime 175951.002500 Acquisition parameters PulseSeq *ep_b0 Protocol dti_35dir PhEncDir COL EchoNo 1 FlipAngle 90 EchoTime 84 InversionTime -1 RepetitionTime 9000 PhEncFOV 256 ReadoutFOV 256 Image information RunNo 15 SeriesNo 16 ImageNo 1 NImageRows 1152 NImageCols 1152 NFrames 35 SliceArraylSize 72 IsMosaic 1 ImgPos 100.2677 150.4042 -45.1647 VolRes 2.0000 2.0000 2.0000 VolDim 128 128 72 Vc -0.9892 -0.0962 0.1107 Vr 0.0985 -0.9950 0.0158 Vs 0.1086 0.0266 0.9937 VolCenter -5.9201 12.6446 42.5760 TransferSyntaxUID 1.2.840.10008.1.2.2 UseSliceScaleFactor 0 (slice 0: 1) sagrev = 0, correv =0, trarev = 0 Vs = 0.108601 0.0265576 0.993731 INFO: no Siemens slice order reversal detected (good!). TR=9000.00, TE=84.00, TI=-1.00, flip angle=90.00 i_ras = (-0.989192, -0.0961755, 0.110675) j_ras = (0.0985118, -0.99501, 0.0158258) k_ras = (0.108601, 0.0265576, 0.993731) writing to /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz... crypt_gkey = FSre8SL5LUSmU mri_probedicom --i /data/bil-mb7/fMRI/MESSI/HC2/testtrac/203/E1S16I1.dcm > /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dcminfo.dat cp testbvecs.txt /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.mghdti.bvecs cp testbvals.txt /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.mghdti.bvals mv -f /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvecs.tmp /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.mghdti.bvecs mv -f /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvals.tmp /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.mghdti.bvals flip4fsl /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig_flip.nii.gz INFO: input image orientation is LPS INFO: input image determinant is 8 fslswapdim /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz x -y z /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig_flip.nii.gz INFO: left-right orientation was flipped by fslswapdim fslorient -forceradiological /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig_flip.nii.gz INFO: found /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.mghdti.bvals, converting to FSL format INFO: found /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.mghdti.bvecs, converting to FSL format mv -f /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig_flip.mghdti.bvecs /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvecs mv -f /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig_flip.mghdti.bvals /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvals eddy_correct /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig_flip.nii.gz /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.nii.gz 0 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0000 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0001 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0002 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0003 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0004 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0005 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0006 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0007 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0008 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0009 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0010 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0011 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0012 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0013 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0014 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0015 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0016 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0017 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0018 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0019 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0020 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0021 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0022 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0023 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0024 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0025 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0026 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0027 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0028 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0029 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0030 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0031 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0032 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0033 processing /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_tmp0034 mv -f /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvecs /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvecs.norot xfmrot /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.ecclog /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvecs.norot /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvecs ln -sf /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.nii.gz /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/data.nii.gz mri_convert --frame 0 1 2 3 4 /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.nii.gz /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb.nii.gz mri_convert --frame 0 1 2 3 4 /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.nii.gz /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb.nii.gz $Id: mri_convert.c,v 1.213 2014/07/29 19:22:31 fischl Exp $ reading from /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.nii.gz... crypt_gkey = FSre8SL5LUSmU TR=1000.00, TE=0.00, TI=0.00, flip angle=0.00 i_ras = (-0.989192, -0.0961755, 0.110675) j_ras = (-0.0985118, 0.99501, -0.0158258) k_ras = (0.108601, 0.0265576, 0.993731) keeping frames 0 1 2 3 4 writing to /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb.nii.gz... crypt_gkey = FSre8SL5LUSmU mri_concat --i /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb.nii.gz --mean --o /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb.nii.gz ninputs = 1 Checking inputs nframestot = 5 Allocing output Done allocing nframes = 5 Computing mean across frames Writing to /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb.nii.gz bet /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb.nii.gz /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb_brain.nii.gz -m -f 0.3 mv -f /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/lowb_brain_mask.nii.gz /home/karunas/Desktop/bin/freesurfer/subjects/203/dlabel/diff #------------------------------------- #@# Image quality assessment Wed Sep 23 12:44:54 PDT 2015 /home/karunas/Desktop/bin/freesurfer/bin/dmri_motion --dwi /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz --mat /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.ecclog --bval /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvals --out /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_motion.txt cwd /data/bil-mb7/fMRI/MESSI/HC2/testtrac/203 cmdline /home/karunas/Desktop/bin/freesurfer/bin/dmri_motion --dwi /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz --mat /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.ecclog --bval /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvals --out /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_motion.txt sysname Linux hostname manas.radiology.ucsf.edu machine x86_64 user karunas Output motion measure file: /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_motion.txt Input transform file: /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi.ecclog Input DWI file: /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz Input b-value table: /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvals Low-b image intensity threshold: 100 Nominal diffusivity: 0.001 Loading volume-to-baseline affine transformations Computing between-volume head motion measures INFO: Processed transforms for 35 volumes Loading DWI volume series from /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/dwi_orig.nii.gz Loading b-value table from /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvals ERROR: Could not open /home/karunas/Desktop/bin/freesurfer/subjects/203/dmri/bvals for reading Linux manas.radiology.ucsf.edu 2.6.18-406.el5 #1 SMP Fri May 1 10:37:57 EDT 2015 x86_64 x86_64 x86_64 GNU/Linux