Dear colleagues,
Provided that the physical interaction between the substrate protein (-30kD) and enzyme protein (-80kD) is too weak to determine by a conventional set of techniques, would that be possible that the two proteins potentially form a complex during crystallization process? My inquiry is whether it’s worth performing crystallization trials of the two proteins together without pre-formed complex, e.g.. by Gel Filtration.
Thanks a lot in advance for sharing your ideas and practical experiences in your successful manipulations.
Have a nice weekend.
Wenhua
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Wenhua Zhang, Ph.D
The Cherfils Lab
LBPA, ENS-Cachan
61 Avenue du Président Wilson
94235 Cachan, France
Tel: +33.01.47.40.59.96
Fax: +33.01.47.40.76.71