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Dear colleagues, Provided that the physical interaction between the substrate protein (-30kD) and enzyme protein (-80kD) is too weak to determine by a conventional set of techniques, would that be possible that the two proteins potentially form a complex during crystallization process? My inquiry is whether it’s worth performing crystallization trials of the two proteins together without pre-formed complex, e.g.. by Gel Filtration. Thanks a lot in advance for sharing your ideas and practical experiences in your successful manipulations. Have a nice weekend. Wenhua -- Wenhua Zhang, Ph.D The Cherfils Lab LBPA, ENS-Cachan 61 Avenue du Président Wilson 94235 Cachan, France Tel: +33.01.47.40.59.96 Fax: +33.01.47.40.76.71 Email: [log in to unmask] <mailto:[log in to unmask]> Lab webpage: Structural Biology of Small GTPases <http://www.lbpa.ens-cachan.fr/version-anglaise/teams/structural-biology-of-small-gtpases/>