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Dear all, sorry for the off-topic question but I would like to assess a putative adenylate cyclase / guanylate cyclase activity in a protein with proposed 'moonlighting activities'. In our hands and assays, the protein behaves as a typical protein kinase, but there are several reports about a guanylate cyclase activity. Thus far, we have checked ATP and GTP hydrolysis by HPLC and analyzed the products. We could find ADP as expected for a kinase and small amounts of AMP, but no cAMP. Same for GTP hydrolysis, which however is not as good a substrate, as one would expect for a kinase. I would now like to verify these results with an independent method. Could someone recommend a robust assay for guanylate and adenylate cyclase activty, that relies on detection of the product? What would be the 'gold standard' here? thanks a lot! Michael