>> Subject: Re: Lipaemia index

>> Dear All,

>> I found a strong correlation between lipaemic index and triglyceride concentration measured on a Roche COBAS 8000 analyser, in a very small experiment using samples spiked with 20% intralipid. See attached chart.

>> Best wishes,

>> Paul

>> Specialty Registrar in Chemical Pathology (Metabolic Medicine)

>> Belfast Health and Social Care Trust Department of Clinical

>> Biochemistry, Kelvin Building, Royal Victoria Hospital Grosvenor

>> Road, Belfast, Northern Ireland, BT12 6BA

>> --------------------------------------------

>> On Wed, 2/9/15, Waller, Paul <[log in to unmask]> wrote:

>> Subject: Re: Lipaemia index

>> Date: Wednesday, 2 September, 2015, 15:34

>> To add my own unpublished

>> observations;

>> Intralipid

>> behaves very differently to endogenous chylomicrons, and can cause apparent interference in kinetic assays. My unproven hypothesis was that this was due to detergents/surfactants in reagents breaking down the lipid vesicles and thus causing a decrease in absorbance due to turbidity.

>> I did not see this phenomenon

>> to the same extent in lipaemic patients sera.

>> Paul Waller

>> MSc CSci FIBMS FHEA

>> Associate Professor in

>> Biomedical Science

>> Associate Head

>> Biomolecular Sciences

>> School of Life

>> Sciences, Pharmacy and Chemistry

>> T 020 8417

>> 7783 (Direct Dial) / 67783 (Internal) E [log in to unmask] /

>> Web page Mr Paul Waller

>> Room PR MB 1019, Penrhyn Road, Kingston upon Thames, KT1 2EE

>> Office

>> hours by appointment

>> -----Original Message-----

>> From: Clinical biochemistry discussion list

>> [mailto:[log in to unmask]]

>> On Behalf Of [log in to unmask]

>> Sent: 02 September 2015 15:24

>> Subject: Re: Lipaemia index

>> Well put, Graham. I was going to reply similarly, but I think you

>> describe very well the variability in chylomicron particle size and their triglyceride content. As I mentioned previously in this thread, several publications and my own unpublished observations show that there is very little correlation between L (or T)-index and triglyceride concentration in lipemic specimens.

>> Note

>> that Soha Zouwail mentioned to me off list that she found good correlation between the triglyceride concentration in Intralipid and L-index. I have used Intralipid to check for lipemia (turbidity) interference, but did not look at the correlation with the L-index. In fact, I think the last time I did such interference studies was before the specimen indices era. I think this makes sense though because the lipid vesicles of Intralipid are likely to be much more uniform than chylomicrons.

>> -Jim

>> -----Original Message-----

>> From: Clinical biochemistry discussion list

>> [mailto:[log in to unmask]]

>> On Behalf Of Graham Jones

>> Sent: Wednesday,

>> September 02, 2015 8:27 AM

>> Subject: Re: Lipaemia index

>> Derreck,

>> I

>> expect not. Rather than having a lot of nice fresh chylomicrons, all the same size and same triglycerides (TG) content (and perhaps the same light scattering), I would expect in any sample we may have a range of difference sizes and TG content (and light scattering) as they lose TG to tissues on their way to the liver. Thus the relationship between TG and light scattering is not very close.

>> Just my thoughts,

>> Graham

>> ________________________________________

>> From: Clinical biochemistry discussion list

>> <[log in to unmask]>

>> on behalf of Mccullough Derreck (UHMB)

>> <[log in to unmask]>

>> Sent: Wednesday, 2 September 2015 6:45 PM

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=

>> Subject: Re: Lipaemia index

>> Hi Jim,

>> As chylomicrons are

>> 90% triglyceride would you not expect a good correlation between triglycerides and turbidity if turbidity is caused by chylomicrons?

>> Bw,

>> Derreck

>> -----Original Message-----

>> From: Clinical biochemistry discussion list

>> [mailto:[log in to unmask]]

>> On Behalf Of [log in to unmask]

>> Sent: 26 August 2015 18:14

>> To:

>> [log in to unmask]

>> Subject: Re: Lipaemia index

>> Replying to myself here! I agree with Alan that Sonia's triglyceride

>> vs L- (or T-index) is much higher than expected. I've had a chance

>> to dig out one of my

>> correlations: Triglyceride, mg/dL = 0.88 L-index + 185, R^2 = 0.017. (Sorry for U.S. units, but the key here is the very low R^2.) My bad that I didn't indicate the instrument/method on the graph, but it was a Roche instrument, probably an Integra. Also, earlier I implied that VLDL, being triglyceride rich, would increase the L/T-index. VLDL rarely does, but most often it is chylomicrons that cause the turbidity.

>> Sonia, you mention that your L-index results are binned, that is, 1+, 2+, etc. yet your graph has numbers for the L-index. Does your Architect give you both the numerical L-index and the binned result?

>> -Jim

>> ------ACB

>> discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.

>> Please

>> note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.

>> ACB Web Site

>> AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdh

>> hwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4

>> Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=

>> Green Laboratories Work

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymed

>> icine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r

>> =_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9

>> khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e

>> =

>> List Archives

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.u

>> k_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHg

>> EZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&

>> m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0yn

>> XNqWBO9iOXZ39XRyBZ8egc&e= List Instructions (How to leave etc.)

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.u

>> k_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TS

>> QgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_

>> XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=

>> ------ACB discussion

>> List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.

>> Please note, archived messages

>> are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.

>> ACB Web

>> Site

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=

>> AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdh

>> hwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4

>> Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=

>> Green Laboratories Work

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymed

>> icine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r

>> =_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9

>> khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e

>> =

>> List Archives

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.u

>> k_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHg

>> EZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&

>> m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0yn

>> XNqWBO9iOXZ39XRyBZ8egc&e= List Instructions (How to leave etc.)

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.u

>> k_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TS

>> QgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_

>> XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=

>> ------ACB discussion

>> List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.

>> Please note, archived messages

>> are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.

>> ACB Web

>> Site

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=

>> AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdh

>> hwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4

>> Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=

>> Green Laboratories Work

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymed

>> icine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r

>> =_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9

>> khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e

>> =

>> List Archives

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.u

>> k_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHg

>> EZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&

>> m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0yn

>> XNqWBO9iOXZ39XRyBZ8egc&e= List Instructions (How to leave etc.)

>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.u

>> k_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TS

>> QgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_

>> XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=

>> ------ACB discussion

>> List Information--------

>> This is an open

>> discussion list for the academic and clinical community working in clinical biochemistry.

>> Please

>> note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.

>> ACB Web Site

>> Green Laboratories Work

>> http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html

>> List Archives

>> List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/

>> ------ACB discussion List

>> Information--------

>> This is an open

>> discussion list for the academic and clinical community working in clinical biochemistry.

>> Please

>> note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.

>> ACB Web Site

>> Green Laboratories Work

>> http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html

>> List Archives

>> List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/

>> ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.

>> Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.

>> ACB Web Site

>> Green Laboratories Work

>> http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html

>> List Archives

>> List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/

>> DISCLAIMER: This email and any files transmitted with it are

>> confidential and intended solely for the use of the individual,

>> individuals or entity to whom they are addressed. Any views or

>> opinions expressed are those of the author and do not necessarily

>> represent the views of Poole Hospital NHS Foundation Trust unless

>> otherwise explicitly stated. This email may contain confidential

>> and/or privileged materials. In the case of confidential and/or

>> privileged materials, any review, retransmission, dissemination or

>> other use of these materials by individuals or entities other than

>> the intended recipient may be the subject of legal action. If you

>> receive an email in error from Poole Hospital NHS Foundation Trust,

>> please contact the sender and delete the material from your computer.

>> ------ACB discussion List Information-------- This is an open

>> discussion list for the academic and clinical community working in clinical biochemistry.

>> Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.

>> ACB Web Site

>> Green Laboratories Work