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Hi Andre Did you go through the practical that I suggested two days ago? It answers your questions 1, 2 (see Page 14). https://wustl.box.com/shared/static/n8pw64dba2tbiridhuzefxrhdmsabdr8.pdf Regarding (3), I am not sure what you mean, this is an ASCII file that you can import in Matlab and treat it as any other array. Cheers Stam On 6 Aug 2015, at 11:36, André Schmidt <[log in to unmask]<mailto:[log in to unmask]>> wrote: Dear fsl experts, I used probtrackx to generate a connectivity matrix for further graph analysis. I used the seed x seed matrix option with 82 seeds. I have a few questions regarding the obtained fdt_network_matrix file: 1) What are the numbers in the txt file exactly describing? 2) Is this matrix weighted to something? 3) How can I modify this matrix to visualize it in matlab? Many thanks for your help. Best wishes André