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Hi Jian, If you had regrouped the individual sections (using the Display GUI), they will all have group-names like group_1, group_2, etc. You could rename the group-names of the second section group2_1, group2_2, etc in order to prevent overlapping groups from the different sections, as these are not necessarily similar in the refined scale-factor (which is the main divider of different groups). HTH, S > Hi Sjors and all, > I have a huge data set. So I divided it into different sections to save > time. After 2D classification, I regroup for each section separately and > sent to auto_refine before 3D classification. Then I want to combine the > auto-refine results of different sections and send to 3D classification. > To generate the combined star file, I just used text editor to modify the > xxx_data.star file by simply copy and paste. My question is how to regroup > this combined star file for 3D classification input? And I know, after > auto_refine, it will regroup it based on more corrected refine result. How > to extract that regroup information from the auto_refine result? How to > combine different sections through relion and regroup them? > Thanks, > Jian > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres