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Hi Vincent, In principle you cannot combine the 3 data sets. The option you mention of re-running the movei-processing step 3x with different sections of the data.star should work. You can do this by just providing a different particles_movie.star file for each movie-refinement: so no need to alter the data.star from the average-particle refinement. You should then also run the polishing in 3 different steps. Hopefully, each of them will reach enough resolution to get good Bfactor and scale factors for all movie frames. If so, then you can combine the polished particles in the end. HTH, Sjors > Hi Sjor, > > I am attempting to do refinement with movie particles (Step 8.3 of > tutorial). I have already done 3D refinement with the average micrographs > previously so I have the _data.star and _optimiser.star files. When I ran > the refinement I got this error: MlModel::expandToMovieFrames ERROR: > unequal number of frames in groupgroup_221 > File: src/ml_model.cpp line: 585 > > I figured this has to do with my dataset being collected in three sessions > and each has different number of frames. They have the same amount of > total > electron dose but the dose per frame is different so the three sessions > have 17, 25, and 29 number frames respectively. > > My question is that is there a way to do movie particles refinement with > all three of these subsets? Can I do the refinement three times using the > same _data.star, each with the two irrelevant subset deleted (for > instance, > run subset 1 with subset 2 and 3 deleted, then run subset 2 with subset 1 > and 3 deleted, etc...)? > > Thank you for your answers, > > Vincent > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres