Hi Zhang, You can do this using fslstats - first, calculate the proportion of 100 voxels relative to the total size of your image: prop = 100 / <total number of voxels in your image> Then use fslstats to find the percentile value at this proportion: fslstats <my_image> -p <100 - 100 * prop> (Use '-a' before '-p' if you are interested in absolute values) You can then use this percentile value as a threshold in fslmaths, to create a mask containing the 100 voxels with the highest intensity: fslmaths <my_image> -thr <threshold_from_fslstats> -bin <my_mask> Cheers, Paul On 7 July 2015 at 07:01, zhang mingxia <[log in to unmask]> wrote: > Hi Iwo, > > I don't want to creat a sphere ROI. I want the 100 voxels with the largest > z value in zstat.nii.gz to be the ROI mask. My question is how to find out > the 100 voxels with largest z value in zstat.nii.gz? > > Thank you again! > > Mingxia Zhang > > On Mon, Jul 6, 2015 at 7:28 PM, Iwo Bohr <[log in to unmask]> wrote: > >> Sorry, one correction to the previous message: the coordinates of the ROI >> central point are expressed in voxels, not in mm! (see below) >> >> Iwo >> >> >> ----- Forwarded Message ----- >> *From:* Iwo Bohr <[log in to unmask]> >> *To:* [log in to unmask] >> *Sent:* Monday, 6 July 2015, 12:22 >> *Subject:* Re: [FSL] making mask of a fixed number of voxels of the most >> responsive ones >> >> In terms of creating your ROI these two commands could be helpful: >> >> 1) fslmaths /usr/local/fsl/data/standard/MNI152_T1_2mm -mul 0 -add 1 -roi >> $c1 1 $c2 1 $c3 1 0 1 ${out}point -odt float >> 2) fslmaths ${out}point -kernel sphere $rad -fmean ${out}$name -odt float >> >> where: >> $c1-$c3 are x,y,z coordinates (voxels) of the centre >> $rad : to replaced by the radius (mm) >> >> The above is for creating a ROI in the MNI space, but it could be >> replaced by any space. >> First command creates the centre of the ROI, second the spherical ROI >> (centred around the point). >> >> Hope this helps, >> >> Iwo >> >> >> >> ------------------------------ >> *From:* zhang mingxia <[log in to unmask]> >> *To:* [log in to unmask] >> *Sent:* Monday, 6 July 2015, 10:39 >> *Subject:* [FSL] making mask of a fixed number of voxels of the most >> responsive ones >> >> Dear FSL experts, >> >> I need to create a mask of the most responsive voxels. Specifically, in >> my study , I got the z value image of one condition (e.g., zstat1.nii.gz). >> Now, I need to create a mask of a fixed number of voxels(e.g., 100) with >> max z value in zstat1.nii.gz. Does any one know how I can do? >> >> Thanks in advance! >> >> Mingxia Zhang >> >> >> >> >> >