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1) Commercially made oligonucleotides
2) M13 rolling circle replication (how people used to make ssDNA for sanger sequencing).

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Dr Antony W Oliver
Senior Research Fellow
CR-UK DNA Repair Enzymes Group
Genome Damage and Stability Centre
Science Park Road
University of Sussex
Falmer, Brighton, BN1 9RQ

e: [log in to unmask]
t(office): +44 (0)1273 678349
t(lab): +44 (0)1273 677512

http://www.sussex.ac.uk/lifesci/oliverlab
http://tinyurl.com/aw-oliver



On 2 Jul 2015, at 16:40, Reza Khayat <[log in to unmask]> wrote:

Hi,
 
Sorry for the non-crystallography question, does anyone know how to produce milligram quantities of single stranded DNA? Thanks.
 
Best wishes,
Reza
 
Reza Khayat, PhD
Assistant Professor
Department of Chemistry
City College of New York
85 Saint Nicholas Terrace, CDI 12318
New York, NY 10031
http://www.khayatlab.org/
212-650-6070