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Hi Almudena,

I'll answer you on the Phenix BB as you've asked specific questions about Phenix tools.

However in general it is a good idea to have a look at your map after phasing and then the (usually two) maps after density modification. If they look like a macromolecule then any of the standard model-building programs should work.

All the best,
Tom T

From: CCP4 bulletin board [[log in to unmask]] on behalf of Almudena Ponce Salvatierra [[log in to unmask]]
Sent: Friday, June 26, 2015 4:32 AM
To: [log in to unmask]
Subject: [ccp4bb] anomalous phasing with HySS - phaser - Autobuild

Hi all, 

I have SeMet data for which HySs locates 127 Selenium atoms with a CC of 0.31, which I think is decent.

Then I run Phaser to generate the first maps, and it gives a score of 25 and LLG of 190 or so...

The next step would be running Autobuild, however, the first models I realize it is building have R work and R free between 50 and 55%. So I guess it is just some random stuff there. 

If the result from HySS and Phaser looks more or less good, why does AutoBuild show these numbers?? Any idea of what I could do to improve so? 

Any ideas are welcome. 

Best wishes, 

Almudena

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Almudena Ponce-Salvatierra
Macromolecular crystallography and Nucleic acid chemistry
Max Planck Institute for Biophysical Chemistry
Am Fassberg 11 37077 Göttingen
Germany