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Does anyone have experience with Tris buffer in cryo protectants? I would expect the pH of the cryosolution to increase a lot during flash freezing which could perhaps destroy the diffraction. I rarely use Tris for crystallization but the current protein really prefers Tris. I would appreciate any comments. Ursula -- Ursula Schulze-Gahmen, Ph.D. Project Scientist UC Berkeley, QB3 360 Stanley Hall #3220 Berkeley, CA 94720-3220 (510) 643 9491