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Hi Kiyotaka and Indrajeet, Sincere thanks to both of you! Now I understand what I edit should be a setting file instead of SPM.mat. Your pointers on global normalization are very enlightening. Yet, the strange thing is, I am not using "proportional" for global normalization. Instead, I am treating it as a covariate by using "ANCOVA". I have attached my saved settings here. Any guess is very much welcomed and appreciated! Thanks a lot, MA