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Looks like a wrong shape for tetrahedral acid
...On May 12, 2015 4:07 PM, "Boaz Shaanan" <[log in to unmask]> wrote:Hi,
As I've just written to David, maybe it's sulfenic acid (result of radiation damage?). I've had those in some cases. See attached scheme for the chemistry and other possibilities for Cys modification by oxidation.
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: [log in to unmask]
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
From: CCP4 bulletin board [[log in to unmask]] on behalf of Bio Physics [[log in to unmask]]
Sent: Tuesday, May 12, 2015 11:57 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] CYS mod
No BME.I have same type of modified CYS !!!Hi All,
On Tue, May 12, 2015 at 2:59 PM, David Schuller <[log in to unmask]> wrote:
So far almost everyone is suggesting BME.
I didn't do the purification & crystallization myself, but I am told:
Buffer was HEPES
cryo was ethylene glycol, glycerol and DMSO.
Some MgSO4 and KPO4
No BME, but maybe some DTT.
Purified from a proteobacterium.
On 05/12/15 15:20, Dyda wrote:
What is in buffer? Perhaps DTT and cacodylate?
Fred
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David J. Schuller
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