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No, it is not sulfenic acid or so, I had that in differend structure. It is L shaped from S, and linear if I put SCX mutaion (another 1.4A way a nice blob). My resolution is 1.3 A. On Tue, May 12, 2015 at 4:18 PM, Artem Evdokimov <[log in to unmask]> wrote: > Looks like a wrong shape for tetrahedral acid > ... > On May 12, 2015 4:07 PM, "Boaz Shaanan" <[log in to unmask]> wrote: > >> Hi, >> >> >> As I've just written to David, maybe it's sulfenic acid (result of >> radiation damage?). I've had those in some cases. See attached scheme for >> the chemistry and other possibilities for Cys modification by oxidation. >> >> Boaz >> >> >> >> >> >> >> >> >> >> >> >> *Boaz Shaanan, Ph.D. Dept. of >> Life Sciences Ben-Gurion University of >> the Negev Beer-Sheva >> 84105 >> Israel >> E-mail: >> [log in to unmask] <[log in to unmask]> Phone: 972-8-647-2220 Skype: >> boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 * >> >> >> >> >> >> ------------------------------ >> *From:* CCP4 bulletin board [[log in to unmask]] on behalf of Bio >> Physics [[log in to unmask]] >> *Sent:* Tuesday, May 12, 2015 11:57 PM >> *To:* [log in to unmask] >> *Subject:* Re: [ccp4bb] CYS mod >> >> >> Hi All, >> >> I have same type of modified CYS !!! >> No BME. >> >> On Tue, May 12, 2015 at 2:59 PM, David Schuller <[log in to unmask]> >> wrote: >> >>> So far almost everyone is suggesting BME. >>> >>> I didn't do the purification & crystallization myself, but I am told: >>> >>> Buffer was HEPES >>> cryo was ethylene glycol, glycerol and DMSO. >>> Some MgSO4 and KPO4 >>> No BME, but maybe some DTT. >>> >>> Purified from a proteobacterium. >>> >>> >>> >>> >>> On 05/12/15 15:20, Dyda wrote: >>> >>>> What is in buffer? Perhaps DTT and cacodylate? >>>> >>>> Fred >>>> >>>> [32m******************************************************************************* >>>> Fred Dyda, Ph.D. Phone:301-402-4496 >>>> Laboratory of Molecular Biology Fax: 301-496-0201 >>>> DHHS/NIH/NIDDK e-mail:[log in to unmask] >>>> Bldg. 5. Room 303 >>>> Bethesda, MD 20892-0560 URGENT message e-mail: >>>> [log in to unmask] >>>> Google maps coords: 39.000597, -77.102102 >>>> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred >>>> ******************************************************************************* >>>> [m >>>> >>> >>> >>> -- >>> ======================================================================= >>> All Things Serve the Beam >>> ======================================================================= >>> David J. Schuller >>> modern man in a post-modern world >>> MacCHESS, Cornell University >>> [log in to unmask] >>> >> >>