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Hi All, I have same type of modified CYS !!! No BME. On Tue, May 12, 2015 at 2:59 PM, David Schuller <[log in to unmask]> wrote: > So far almost everyone is suggesting BME. > > I didn't do the purification & crystallization myself, but I am told: > > Buffer was HEPES > cryo was ethylene glycol, glycerol and DMSO. > Some MgSO4 and KPO4 > No BME, but maybe some DTT. > > Purified from a proteobacterium. > > > > > On 05/12/15 15:20, Dyda wrote: > >> What is in buffer? Perhaps DTT and cacodylate? >> >> Fred >> >> [32m******************************************************************************* >> Fred Dyda, Ph.D. Phone:301-402-4496 >> Laboratory of Molecular Biology Fax: 301-496-0201 >> DHHS/NIH/NIDDK e-mail:[log in to unmask] >> Bldg. 5. Room 303 >> Bethesda, MD 20892-0560 URGENT message e-mail: >> [log in to unmask] >> Google maps coords: 39.000597, -77.102102 >> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred >> ******************************************************************************* >> [m >> > > > -- > ======================================================================= > All Things Serve the Beam > ======================================================================= > David J. Schuller > modern man in a post-modern world > MacCHESS, Cornell University > [log in to unmask] >