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You can generate the template using as many or as few subjects as you like; however, if you have multiple groups and your template contains only subjects from one group, a reviewer will likely raise an issue.

There are two elements to DARTEL:
(1) Template generation;
(2) Creation of the deformation fields.

The later allows you to add more subjects to the analysis without having to regenerate the template set again.



Best Regards, Donald McLaren
=================
D.G. McLaren, Ph.D.
Research Fellow, Department of Neurology, Massachusetts General Hospital and
Harvard Medical School
Postdoctoral Research Fellow, GRECC, Bedford VA
Website: http://www.martinos.org/~mclaren
Office: (773) 406-2464
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On Wed, Apr 8, 2015 at 12:22 PM, Amelie Amelie <[log in to unmask]> wrote:
Dear Donald,

Thanks very much for your reply! So if I use a DARTEL template for preprocessing of functional and anatomical data, I assume I will have to make sure that the subjects that are included in preprocessing are exactly the same ones whose anatomical images are used for creating the DARTEL template? It doesn't seem to be possible to apply a DARTEL template based on a whole group of subjects to a subgroup's preprocessing?

Many thanks again!

Best wishes,
Jingting

On Wed, Apr 8, 2015 at 10:57 AM, MCLAREN, Donald <[log in to unmask]> wrote:
Template_6 is not in MNI space, which is why the DARTEL needs to be normalized to MNI space for all analyses.

When you apply the deformations, make sure that your functional data are aligned to the structural images that were used for DARTEL.

Best Regards, Donald McLaren
=================
D.G. McLaren, Ph.D.
Research Fellow, Department of Neurology, Massachusetts General Hospital and
Harvard Medical School
Postdoctoral Research Fellow, GRECC, Bedford VA
Website: http://www.martinos.org/~mclaren
Office: (773) 406-2464
=====================
This e-mail contains CONFIDENTIAL INFORMATION which may contain PROTECTED
HEALTHCARE INFORMATION and may also be LEGALLY PRIVILEGED and which is
intended only for the use of the individual or entity named above. If the
reader of the e-mail is not the intended recipient or the employee or agent
responsible for delivering it to the intended recipient, you are hereby
notified that you are in possession of confidential and privileged
information. Any unauthorized use, disclosure, copying or the taking of any
action in reliance on the contents of this information is strictly
prohibited and may be unlawful. If you have received this e-mail
unintentionally, please immediately notify the sender via telephone at (773)
406-2464 or email.

On Wed, Apr 8, 2015 at 8:06 AM, Amelie Amelie <[log in to unmask]> wrote:
Dear SPMers,

I intend to create a DARTEL template (gm and wm) in SPM12 based on a good number of experimental subjects so as to replace the MNI template with the DARTEL template in preprocessing of the fMRI data (for univariate analysis). In this case, I assume I just need to do 'Run DARTEL template' but not the 'Normalize to MNI space' step (which is required to do for a VBM analysis)? My understanding is that the Template_6.nii is already in MNI space after 'Run DARTEL Template'. Also in my preprocessing analysis, in the 'normalization' step, I will apply the DARTEL template (deformation parameters) to each subject's images anyway.

Thank you!

Best,
Jingting