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Hello everyone, I'm running TBSS analyses, and was wondering how people are efficiently thresholding their skeletons created after tbss_3_postreg. Situation: Looking at my mean_FA_skeleton, there are roots that wrap around the side of the brain, almost like a halo. It's not a full ring, but I'm not sure if this should be present, or if I'm doing a bad job with previous steps betting/using masks that are too large, and will mess with tbss_4 thresholding and randomise analysis. More focal question: Since we are looking at white matter in DTI, would it be wise to more tightly bet when running topup/eddy/bet results prior to dtifit/tbss? I don't want to be thresholding too highly during tbss_4 if there are better methods that can be used prior to this step, such that I'm not missing out on any significant voxels during the stats sections. Thank you, Kevin Japardi