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Dear Smith, You do not need relion for fitting with Coot or Refmac, and a Windows implementation of the latter should not be a problem. Detailed information on how to fit models into cryo-EM maps are on Garb's website: http://www2.mrc-lmb.cam.ac.uk/groups/murshudov/ HTH, S > Dear All, > > I know from a source it needs relion to work for fit by Coot and Refmac. > Does it mean I cannot do the fitting by the Windows version of Coot and > refmac? > > Is any step by step tutiorial (from a mrc file and a preliminary pdb file) > on doing the fit and refinement by Coot and Refmac? > > I am looking forward to getting your reply. > > Smith -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres