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Thank you for reply. In the fMRI data, if there are two condition than the design matrix X will have the 2 columns. For e.g., in the SPM auditory data set, design matrix X has two columns because it has only two conditions. Thus, number of b's would be 2 only. but HRF has more number of taps than 2. Than how the b would be the amplitude of HRF in that case? Should i form the new design matrix (which should have more number of columns)? Thanks & Warm Regards, Priya