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This is a numerical instability that used to occasionally occur in part of the algorithm. I haven't seen it for a while though. Which spm version are you using? If it is SPM8, do you have the latest updates installed? To save memory, only single precision floating point is used in the computations. This makes algorithms very susceptible to unexpected behaviour when, for example, values are added together in a different order. Best regards, John Bela Bhatia <[log in to unmask]> wrote: >Thanks for the help earlier. > >I am trying to create a Dartel template (sample - 5 subjects) > >The template is good with grey and white matter..... > >When I tried with GM, WM and CSF, the template that spun out had missing brain in the center and a 1/3rd of the images(coronal and sagittal) had a horizontal bar across the bottom 4th of the template