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Dear FSL experts, I have a small group of healthy volunteers scanned twice under 2 different conditions. We project a visual stimulus formed by 2 type of images (image1, image2). In the first run subjects have to look a visual stimulus and the second run they have to count the image2. My EVs are image1 and image2 for both the runs and I am interested to calculated their Activation and Deactivation. I use paired t test to calculate the run1-run2 paired mean difference. I follow the manual https://fsl.fmrib.ox.ac.uk/fsl/fslwiki/FEAT/UserGuide#Paired_Two-Group_Difference_.28Two-Sample_Paired_T-Test.29 I feed feat directories into the second level analysis. I would like to distinguish Activation and Deactivation of my two regressors in paired t test. How can I do? How can i set contrast masking? Thanks for any advice. Anna