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Ronnie

I see a lot of cheap and expensive microscopes, and I notice that expensive is not always better for protein crystallization.

Almost the most important thing is that illumination comes from one particular direction.  Often this means that the light source is small and far from the sample stage.  What does not work well is to have a large light source (eg multiple LEDs, large white screen, mirror or sintered transparent sheet) that is close to the sample - even with the best optics in the world, you won't see crystals well.

Dark ground (see only scattered light) and ordinary transmission mode can both work well - good to have both if possible.

Good luck,

Patrick




On 27 March 2015 at 13:08, Ronnie Berntsson <[log in to unmask]> wrote:
Dear all,

I’m currently looking in to buying a new microscope for viewing crystal plates, mounting crystals etc, and would love some input into what I should get.

What I would like is a microscope that has a high quality image, that is easy to work with and which is ergonomical. It does not have to have a fixed digital camera, but it should be possible to attach a digital camera to take pictures. Price is obviously also important...

I’ve been looking at the standard microscopes that Molecular Dimensions sell, and also on a Nikon SMZ18. I remember that we used to have a Leica microscope in a previous lab that I liked, but can’t seem to find the model at the moment.

I am also interested in getting a UV source, to inspect crystals under UV to see if you fluoresce (and hence are protein crystals). Molecular Dimension used to sell XtalLight 100, but doesn’t seem to do so anymore. Do any of you have other suggestions regarding the possibility of adding UV to a stereomicroscope?

Suggestions and thoughts are more than welcome!

Thanks,
Ronnie


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Ronnie Berntsson, PhD
Assistant Professor
Department of Medical Biochemistry and Biophysics
Umeå University
90187 Umeå
Sweden

e-mail: [log in to unmask]



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