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Hi Steve I have one more comment for this thread. The microbatch-under-oil method is very handy for anaerobic work: 1. You can keep the microbatch stock solutions in normal microtitre plates (polypropylene is best to reduce evaporation) for months, which hugely reduces the amount of degassing that you need to do. You will only use say 0.5 ul of stock per drop. 2. The oil offers a surprising amount of protection from oxidation, which may be helpful eg in harvesting. 3. Microbatch can be automated - in parallel to vapor diffusion if desired It's amazing how often (aerobic) microbatch produces far superior crystals to V.D. for no obvious reason - it's well worth trying for both screening and optimization. Best wishes Patrick On 11 March 2015 at 10:17, <Stephen Carr> <[log in to unmask]> wrote: > Dear CCP4BBer's > > Apologies for the off-topic post, but the CCP4BB seems to be the best > place to ask about crystallisation. > > I am looking to set up crystallisation in an anaerobic glove box and > wondered how other people did this, specifically the crystallisation > stage. My initial thoughts were to place a small crystallisation incubator > inside the box, however the smallest I have come across so far (~27L) is > still rather large. Has anyone come across smaller incubators? > Alternatively are incubators even neccessary if the glove box is placed in > a room with good air conditioning and stable temperature control? > > Any recommendations would be very helpful. > > Thanks in advance, > > Steve Carr > > Dr Stephen Carr > Research Complex at Harwell (RCaH) > Rutherford Appleton Laboratory > Harwell Oxford > Didcot > Oxon OX11 0FA > United Kingdom > Email [log in to unmask] > tel 01235 567717 > > This email and any attachments may contain confidential, copyright and or > privileged material, and are for the use of the intended addressee only. If > you are not the intended addressee or an authorized recipient of the > addressee, please notify us of receipt by returning the e-mail and do not > use, copy, retain, distribute or disclose the information in or attached to > this email. > > Any views or opinions presented are solely those of the author and do not > necessarily represent those of the Research Complex at Harwell. > > There is no guarantee that this email or any attachments are free from > viruses and we cannot accept liability for any damage which you may sustain > as a result of software viruses which may be transmitted in or with the > message. > > We use an electronic filing system. Please send electronic versions of > documents, unless paper is specifically requested. > > This email may have a protective marking, for an explanation, please see: > > http://www.mrc.ac.uk/About/informationandstandards/documentmarking/index.htm > . > -- [log in to unmask] Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36