On Fri Feb 06 2015 at 11:02:12 AM Kerff Fred <[log in to unmask]> wrote:

Hello,

Looking at structure 2HR0 ("The structure of complement C3b provides insights into complement activation and regulation. »,Abdul Ajees, A.,  Gunasekaran, K.,  Volanakis, J.E.,  Narayana, S.V.,  Kotwal, G.J.,  Krishna Murthy, H.M.;  (2006) Nature 444: 221-225), I noticed the absence of contacts between layers in the crystal. Is it something that has already been observed in other crystals?

Best regards,

Fred
-----
Frédéric Kerff
Chercheur qualifié F.R.S.-FNRS
Cristallographie des protéines
Centre d'Ingénierie des Protéines
Université de Liège
17, Allée du 6 Août - Bat B5a
4000 Liège (Belgium)
Tel.: +32 (0)4 3663620
Fax: +32 (0)4 3663772



> Le 6 févr. 2015 à 10:12, Tim Gruene <[log in to unmask]> a écrit :
>
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> Dear Smith,
>
> The sca file most likely does not contain flags. pointless can read
> the sca file, standardise it to ccp4 standards and freerflag marks
> random reflections. You should use the maximum of 500 unique
> reflections or 5% of the unique reflections, whichever is larger.
>
> Best,
> Tim
>
> On 02/06/2015 09:49 AM, Smith Lee wrote:
>> Dear All, I have a sca file. Will you please tell me by which
>> software or how I can know whether the sca file contains R-free
>> tags? If not, by which software or how I can add the R-free tags?
>> And how much of the reflections I add the R-free tags? I am looking
>> forward to getting your reply. Smith
>>
>
> - --
> - --
> Dr Tim Gruene
> Institut fuer anorganische Chemie
> Tammannstr. 4
> D-37077 Goettingen
>
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