Haven'tthat paper and the associated structure been retracted?
http://www.nature.com/news/2009/091222/full/462970a.html
There was a huge scandal when it was discovered that Krishna Murthy had
falsified data, including the structure you refer to.
See http://en.wikipedia.org/wiki/H.M._Krishna_Murthy
A crystallographer with a wikipedia entry for all the wrong reasons...
Dave
On Fri Feb 06 2015 at 11:02:12 AM Kerff Fred <[log in to unmask]> wrote:
> Hello,
>
> Looking at structure 2HR0 ("The structure of complement C3b provides
> insights into complement activation and regulation. »,Abdul Ajees, A.,
> Gunasekaran, K., Volanakis, J.E., Narayana, S.V., Kotwal, G.J., Krishna
> Murthy, H.M.; (2006) Nature 444: 221-225), I noticed the absence of
> contacts between layers in the crystal. Is it something that has already
> been observed in other crystals?
>
> Best regards,
>
> Fred
> -----
> Frédéric Kerff
> Chercheur qualifié F.R.S.-FNRS
> Cristallographie des protéines
> Centre d'Ingénierie des Protéines
> Université de Liège
> 17, Allée du 6 Août - Bat B5a
> 4000 Liège (Belgium)
> Tel.: +32 (0)4 3663620
> Fax: +32 (0)4 3663772
>
>
>
> > Le 6 févr. 2015 à 10:12, Tim Gruene <[log in to unmask]> a écrit :
> >
> > -----BEGIN PGP SIGNED MESSAGE-----
> > Hash: SHA1
> >
> > Dear Smith,
> >
> > The sca file most likely does not contain flags. pointless can read
> > the sca file, standardise it to ccp4 standards and freerflag marks
> > random reflections. You should use the maximum of 500 unique
> > reflections or 5% of the unique reflections, whichever is larger.
> >
> > Best,
> > Tim
> >
> > On 02/06/2015 09:49 AM, Smith Lee wrote:
> >> Dear All, I have a sca file. Will you please tell me by which
> >> software or how I can know whether the sca file contains R-free
> >> tags? If not, by which software or how I can add the R-free tags?
> >> And how much of the reflections I add the R-free tags? I am looking
> >> forward to getting your reply. Smith
> >>
> >
> > - --
> > - --
> > Dr Tim Gruene
> > Institut fuer anorganische Chemie
> > Tammannstr. 4
> > D-37077 Goettingen
> >
> > GPG Key ID = A46BEE1A
> >
> > -----BEGIN PGP SIGNATURE-----
> > Version: GnuPG v1.4.12 (GNU/Linux)
> >
> > iD8DBQFU1IWVUxlJ7aRr7hoRAmZHAJ4+6wREnwkFN0EhfErAA0tPSopKKwCgiLdi
> > j0JFZac4kAh8twpov71MG84=
> > =XN57
> > -----END PGP SIGNATURE-----
>
