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...And the cryoprotectant was simply the Li/NH4SO4 mother liquor. Also maybe relevant: the crystals were pretty old, a year or so. JPK -----Original Message----- From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Keller, Jacob Sent: Monday, February 23, 2015 12:21 PM To: [log in to unmask] Subject: [ccp4bb] Blob Game Dear Crystallographers, I've got a strange blob hanging off an arginine--see attached. Any ideas? Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2, NaCl, Li2SO4. There are really no other side chains which could be close enough, and there's nothing in the anomalous LLG map. ??? JPK ******************************************* Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: [log in to unmask] *******************************************